The absolute OD and the relative OD were determined using AIWBwell analysis software
This discrepancy could be due to the different parasite strains used (BinH vs
Jain, A., et al.,p62/SQSTM1 is a target gene for transcription factor NRF2 and creates a positive feedback loop by inducing antioxidant response element-driven gene transcription
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Western blot analysis Total protein was extracted with RIPA buffer (Pierce, 89900) supplemented with protease inhibitor (Life Technologies, 1861280) and quantified using the BCA Protein Assay (Thermo Scientific)
While only the L isomer is present in the biologic system, commercial synthesis of carnitine produces a D,L racemic mixture, from which the L-isomer is obtained